Detection and genotyping of human papillomavirus in gynaecologic outpatients of Messina, eastern Sicily, Italy

作者：Giuffre Giuseppe^*; Simone Angela; Todaro Paolo; Le Donne Maria; Caruso Carmela; Pizzo Alfonsa; Granese Domenico

来源：Oncology Reports, 2010, 23(3): 745-750.

DOI：10.3892/or_00000693

• HPV infection
• genotypes
• prevalence
• Sicily
• Italy

In order to determine the prevalence of human papillomavirus (HPV) infection in sexually active female population in Messina, we tested cervical scrapes of women referred to university clinics for routine gynaecologic care. Between March and December 2008, a total of 680 cervical samples of 598 patients (573 Italian from province of Messina and 25 resident aliens) were examined consecutively from laboratory of molecular biology at the Department of Human Pathology. For each sample, cervical cells were collected by centrifugation and DNA was extracted (QlAamp DNA mini kit, Qiagen), followed by a PCR-based HPV DNA assay and reverse clot blot genotyping (HPV-HS Bio plus HPV-strip, Analytica or HPV-type, AB Analytica). The overall rate of HPV DNA detection in Italian patients (mean age 34 years; range 15-69) was 70.5% (404/573). with 163 cases of multiple infections (40.3%). In 335 patients (82.9%) a high-risk HPV infection was detected. In this group) the coexistence of a low-risk HPV infection was documented ill 97 cases while 65 patients exhibited only a low-risk HPV infection. HPV-16 was the most prevalent (33.4%), followed by HPV-6 (28.0%). HPV-31 (24.3%), HPV-58 (11.4%), HPV-66 (11.1%), HPV-53 (6.4%), HPV-18 (6.2%), HPV-56 (5.4%), HPV-33 (5.2%) while the other genotypes identified (HPV-11, -40, -42, -43, -44, -54, -61, -70, -81 -26, -35, -39, -45, -51, -52, -59, -68, -73, -82) were below 5%. HPV prevalence (any type) was 79.7% at age <= 24 years, 73.4% at 25-34 years and 67.1% at 35-44 years and 58.1% at age >= 45 years. A significant association (chi(2)=12.718; P=0.006) between HPV DNA detection and the younger age was encountered. Since available data oil the prevalence and distribution of HPV infection ill Italy are somewhat discordant, this study represents a helpful contribution to the knowledge oil the circulation of precise genotypes in east Sicily in order to improve new HBV vaccines.

• 出版日期2010-3