Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects

作者：Medeiros Lea A; Dennis Lucas M; Gill Mark E; Houbaviy Hristo; Markoulaki Styliani; Fu Dongdong; White Amy C; Kirak Oktay; Sharp Phillip A; Page David C; Jaenisch Rudolf^*

来源：Proceedings of the National Academy of Sciences, 2011, 108(34): 14163-14168.

DOI：10.1073/pnas.1111241108

Mir-290 through mir-295 (mir-290-295) is a mammalian-specific microRNA (miRNA) cluster that, in mice, is expressed specifically in early embryos and embryonic germ cells. Here, we show that mir-290-295 plays important roles in embryonic development as indicated by the partially penetrant lethality of mutant embryos. In addition, we show that in surviving mir-290-295-deficient embryos, female but not male fertility is compromised. This impairment in fertility arises from a defect in migrating primordial germ cells and occurs equally in male and female mutant animals. Male mir-290-295(-/-) mice, due to the extended proliferative lifespan of their germ cells, are able to recover from this initial germ cell loss and are fertile. Female mir-290-295(-/-) mice are unable to recover and are sterile, due to premature ovarian failure.

• 出版日期2011-8-23