MPEP reduces seizure severity in Fmr-1 KO mice over expressing human A beta

作者：Westmark Cara J^*; Westmark Pamela R; Malter James S

来源：International Journal of Clinical and Experimental Pathology, 2010, 3(1): 56-68.

• APP
• beta-amyloid
• FMRP
• FRAXAD
• PTZ
• seizure
• Tg2576

Metabotropic glutamate receptor 5 (mGluR(5)) regulates the translation of amyloid precursor protein (APP) mRNA. Under resting conditions, mRNA is bound to and translationally repressed by the fragile X mental retardation protein (FMRP). Upon group 1 mGluR activation, FMRP dissociates from the mRNA and translation ensues. APP levels are elevated in the dendrites of primary neuronal cultures as well as in synaptoneurosomes (SN) prepared from embryonic and juvenile fmr-1 knockout (KO) mice, respectively. In order to study the effects of APP and its proteolytic product A beta on Fragile X syndrome (FXS) phenotypes, we created a novel mouse model (FRAXAD) that over-expresses human APP(Swe)/A beta in an fmr-1 KO background. Herein, we assess (1) human APP(Swe) and A beta levels as a function of age in FRAXAD mice, and (2) seizure susceptibility to pentylenetetrazol (PTZ) after mGluR(5) blockade. PTZ-induced seizure severity is decreased in FRAXAD mice pre-treated with the mGluR(5) antagonist MPEP. These data suggest that A beta contributes to seizure incidence and may be an appropriate therapeutic target to lessen seizure pathology in FXS, Alzheimer's disease (AD) and Down syndrome (DS) patients.

• 出版日期2010