Apoptotic Effect of Quercetin on HT-29 Colon Cancer Cells via the AMPK Signaling Pathway

作者：Kim Hyeong Jin; Kim Sang Ki; Kim Byeong Soo; Lee Seung Ho; Park Young Seok; Park Byung Kwon; Kim So Jung; Kim Jin; Choi Changsun; Kim Jong Suk; Cho Sung Dae; Jung Ji Won; Roh Kyong Hwan; Kang Kyung Sun; Jung Ji Youn^*

来源：Journal of Agricultural and Food Chemistry, 2010, 58(15): 8643-8650.

DOI：10.1021/jf101510z

• Quercetin
• HT-29
• AMPK
• p53
• p21
• apoptosis
• cancer
• chemoprevention

Activation of AMP-activated protein kinase (AMPK), a physiological cellular energy sensor, strongly suppresses cell proliferation in both nonmalignant and tumor cells. This study demonstrates the mechanism of quercetin-induced apoptosis in HT-29 colon cancer cells. Treatment of cells with quercetin significantly decreased cell viability in a dose-dependent manner. Notably, quercetin increased cell cycle arrest in the G1 phase and up-regulated apoptosis-related proteins, such as AMPK, p53, and p21, within 48 h. Furthermore, in vivo experiments showed that quercetin treatment resulted in a significant reduction in tumor volume over 6 weeks, and apoptosis-related protein induction by quercetin was significantly higher in the 100 mg/kg treated group compared to the control group. All of these results indicate that quercetin induces apoptosis via AMPK activation and p53-dependent apoptotic cell death in HT-29 colon cancer cells and that it may be a potential chemopreventive or therapeutic agent against HT-29 colon cancer.

• 出版日期2010-8-11
• 单位河北医科大学