Acute renal failure during sepsis: Potential role of cell cycle regulation

作者：Yang Quan hui; Liu Da wei^*; Long Yun; Liu Hong zhong; Chai Wen zhao; Wang Xiao Ting

来源：Journal of Infection, 2009, 58(6): 459-464.

DOI：10.1016/j.jinf.2009.04.003

• Renal failure
• Acute
• Cyclin
• Cyclin-dependent kinase
• Cyclin-dependent kinase inhibitor

Objectives: This study aimed to evaluate cell cycle regulation in acute kidney injury after intraperitoneal sepsis in rats.
Methods: Polymicrobial. sepsis was induced by cecal ligation and puncture (CLP) in rats. At 0, 6, 12, 24, 48, and 72 h after CLP, serum creatinine was evaluated. DNA content of isolated kidney cells was analyzed using flow cytometer. Furthermore, the expression of p21, p53, cyclin D1, cyclin E, CDK2, CDK4 and P-pRb was also measured by western blot.
Results: After sepsis-induced by CLP, kidney injury of rat was associated with G1 cell cycle arrest, however, recovery of renal function related to cell cycle progression 48 h after CLP. Results also showed that the upregulation of p53 and p21 was correlated with G1 cell arrest in 48 h after CLP. Nevertheless, upregulation of cyclin D1/CDK4 and cyclin E/CDK2 induced pRb phosphorylation, which resulted in the G1/S transition 48 h after CLP.
Conclusion: The data suggest that G1 cell cycle arrest may play a role in the initiation of kidney injury, whereas, through regulating cell cycle, p53, p21, CDKs, cyclins and P-pRb may be involved in the injury or recovery of renal function after intraperitoneal sepsis.

• 出版日期2009-6
• 单位北京协和医学院; 中国医学科学院北京协和医院