A Meal High in Saturated Fat Evokes Postprandial Dyslipemia, Hyperinsulinemia, and Altered Lipoprotein Expression in Obese Children With and Without Nonalcoholic Fatty Liver Disease

作者：Mager Diana R^*; Mazurak Vera; Rodriguez Dimitrescu Carla; Vine Donna; Jetha Mary; Ball Geoff; Yap Jason

来源：Journal of Parenteral and Enteral Nutrition, 2013, 37(4): 517-528.

DOI：10.1177/0148607112467820

• postprandial
• lipid
• lipoprotein
• children
• nonalcoholic fatty liver disease

Background: Hyperinsulinemia and altered lipid and lipoprotein metabolism induced by fast-food diets may contribute to nonalcoholic fatty liver disease (NAFLD). We hypothesized that a high saturated fat (SFA) meal would evoke prolonged postprandial lipemia and hyperinsulinemia, increased inflammation, and altered lipoprotein expression in obese children with NAFLD when compared with healthy children. Methods: We prospectively studied 31 children (NAFLD, 13.1 +/- 2.6 years, n = 11; age-matched obese, 14.3 +/- 1.7 years, n = 9; lean, 13.6 +/- 2.6 years, n = 11) following consumption of a high SFA (18.8%) meal. Prior to and at 1, 3, and 6 hours after meal consumption, blood was collected for analysis of alanine aminotransferase (ALT); aspartate aminotransferase (AST); gamma-glutamyltransferase; leptin; C-reactive protein; (fasting) insulin; glucose; triglycerides (TGs); total, high-density lipoprotein, and low-density lipoprotein cholesterol; adiponectin; nonesterified fatty acids (NEFAs); inflammatory markers (TNF-alpha, IL-6, IL-10); apolipoproteins-B48, B100, and CIII; and fatty acid (FA) composition of TG fractions. Results: Children with NAFLD had significantly higher fasting levels of ALT (87 +/- 54 U/L), AST (52 +/- 33.5 U/L), and apolipoprotein-CIII (20.6 +/- 11.3 mg/dL) with postprandial hyperinsulinemia (iAUC insulin: 225 +/- 207 [NAFLD] vs 113 +/- 73 [obese] vs 47 +/- 19.9 [lean] mU/L-h; P < .001); suppression of NEFA (iAUC-NEFA: 1.7 +/- 0.9 [NAFLD] vs 0.6 +/- 0.3 [obese] vs 1 +/- 0.7 [lean] mEq/L-h); and prolonged elevations in apolipoprotein-B48 3-6 hours after meal consumption when compared with obese and lean controls (P < .05). Conclusion: A meal high in saturated fat evokes postprandial dyslipemia, hyperinsulinemia, and altered lipoprotein expression in obese children with and without NAFLD.

• 出版日期2013-7