NO ASSOCIATION BETWEEN SUSCEPTIBILITY TO MULTIPLE-SCLEROSIS AND HLA-DPB1 ALLELES IN THE FRENCH-CANADIAN POPULATION

作者：HOWELL WM^*; SAGE DA; EVANS PR; SMITH JL; FRANCIS GS; HAEGERT DG

来源：Tissue Antigens, 1991, 37(4): 156-160.

DOI：10.1111/j.1399-0039.1991.tb01864.x

• FRENCH CANADIAN
• HLA-DP
• MULTIPLE SCLEROSIS
• PCR-SSOP

HLA-DPB1 typing was performed using polymerase chain reaction DNA amplification and sequence-specific oligonucleotide probing (PCR-SSOP) which permitted identification of 17 distinct DPB alleles using 15 oligonucleotide probes. The accuracy of this approach was confirmed in an initial study of 26 human B-lymphoblastoid cell lines which demonstrated close agreement between PCR-SSOP and PLT assigned types. A cohort of 47 adult French Canadians was then studied to provide an estimate of DPB1 allelic frequencies in an ethnically homogeneous population. DPB1*0401 was the most frequent phenotype (61.5%) and only DPB1*0101, 0301 and 0402 were also present at frequencies greater than 10%. HLA-DPw4 has been reported to be associated with multiple sclerosis (MS) but our PCR-SSOP analysis of 52 French Canadian MS patients showed no association with either the DPB1*0401 or DPB1*0402 splits of DPw4 or with any other DPB1 allele.

• 出版日期1991-4