Murine bone marrow-derived DCs activated by porcine rotavirus stimulate the Thl subtype response in vitro

作者：Ye, Liping; Jiang, Yanlong; Yang, Guilian; Yang, Wentao; Hu, Jingtao; Cui, Yulin; Shi, Chunwei; Liu, Jing; Wang, Chunfeng^*

来源：Microbial Pathogenesis, 2017, 110: 325-334.

DOI：10.1016/j.micpath.2017.07.015

• Rotavirus
• Bone marrow-derived DCs (BMDCs)
• Activation

Rotavirus (RV) infection causes acute, watery dehydrating diarrhea and even death in infants and other young animals, resulting in a severe economic burden; however, little is known about the innate immune mechanisms associated with RV infection. Dendritic cells (DCs), which are professional antigen presenting cells (APCs), serve as a bridge connecting the innate and adaptive immune system. In this study, the interaction between murine bone marrow-derived DCs (BMDCs) and porcine rotavirus (PRV) was investigated in vitro. Upon stimulation, the expression levels of MHC-II, CD40, CD80, CD86 and CD83 in BMDCs increased in a time-dependent manner, indicating activation and maturation by PRV. In addition, up-regulated Toll-like receptor 2 (TLR2), TLR3 and NF-kappa B increased the production of interleukin-12 and interferon-gamma. The PRV-stimulated BMDCs also showed increased stimulatory capacity in mixed lymphocyte reactions and promoted the Thl subtype response.

• 出版日期2017-9
• 单位吉林农业大学