Multiple spectroscopic studies on the interaction between olaquindox, a feed additive, and bovine serum albumin

作者：Xu Tianci; Guo Xingjia; Zhang Lei^*; Pan Fang; Lv Junna; Zhang Yunyu; Jin Hongjing

来源：Food and Chemical Toxicology, 2012, 50(7): 2540-2546.

DOI：10.1016/j.fct.2012.04.007

• Olaquindox
• Bovine serum albumin
• Interaction
• Fluorescence quenching
• Three-dimensional fluorescence spectroscopy

The interaction between olaquindox (OLA) and bovine serum albumin (BSA) was investigated using fluorescence, UV-vis absorption and circular dichroism (CD) spectroscopy. The results showed that the fluorescence quenching of BSA by OLA was a static quenching process induced by the formation of OLA-BSA complex. The binding constant of OLA-BSA complex was calculated to be 1.299 x 10(4) L mol(-1) (293 K). The negative values of Delta H degrees and Delta S degrees indicated that hydrogen bond and van der Waals interactions played major roles in stabilizing the complex. Site probe competition experiments and number of binding sites (n) revealed that OLA could bind to site I in subdomain IIA of BSA, and the binding distance (r) was evaluated to be 3.643 nm according to Forster's non-radiative energy transfer theory. The results of CD and three-dimensional fluorescence spectra suggested some conformational changes of BSA after OLA binding.

• 出版日期2012-7
• 单位辽宁大学