Aging of porous silicon in physiological conditions: Cell adhesion modes on scaled 1D micropatterns

作者：Munoz Noval Alvaro; Sanchez Vaquero Vanessa; Punzon Quijorna Esther; Torres Costa Vicente; Gallach Perez Dario; Gonzalez Mendez Laura; Montero Isabel; Martin Palma Raul J; Climent Font Aurelio; Garcia Ruiz Josefa P; Manso Silvan Miguel

来源：Journal of Biomedical Materials Research Part A, 2012, 100A(6): 1615-1622.

DOI：10.1002/jbm.a.34108

• porous silicon
• physiological aging
• ion-beam modification
• mesenchymal stem cells
• cell guides

The surface properties of porous silicon (PSi) evolve rapidly in phosphate-buffered saline. X-ray photoelectron spectra indicate the formation of a SiOH and CO enriched surface, which becomes increasingly hydrophilic with aging time. Multiscale stripe micropatterns of Si and PSi have been fabricated by means of a high-energy ion-beam irradiation process. These micropatterns have been aged in physiological conditions and used to analyze human mesenchymal stem cell (hMSC) adhesion. The actin cytoskeleton of hMSCs orients following the uniaxial micropatterns. In the wider Si stripes, hMSCs are dominantly located on Si areas. However, for reduced Si widths, adhesion is avoided on PSi by a split assembly of the actin cytoskeleton on two parallel Si areas. These results confirm that nanostructured SiOH/CO-rich surfaces with hydrophilic character are specially adapted for the creation of cell adhesion surface contrasts.

• 出版日期2012-6