A whole-genome assembly of Drosophila

作者：Myers EW^*; Sutton GG; Delcher AL; Dew IM; Fasulo DP; Flanigan MJ; Kravitz SA; Mobarry CM; Reinert KHJ; Remington KA; Anson EL; Bolanos RA; Chou HH; Jordan CM; Halpern AL; Lonardi S; Beasley EM; Brandon RC; Chen L; Dunn PJ; Lai ZW; Liang Y; Nusskern DR; Zhan M; Zhang Q; Zheng XQ; Rubin GM; Adams MD; Venter JC

来源：Science, 2000, 287(5461): 2196-2204.

DOI：10.1126/science.287.5461.2196

We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9-megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99.99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.

• 出版日期2000-3-24