Fasting time duration modulates the onset of insulin-induced hypoglycemic seizures in mice

作者：Pitchaimani Vigneshwaran; Arumugam Somasundaram; Thandavarayan Rajarajan Amirthalingam; Karuppagounder Vengadeshprabhu; Afrin Mst Rejina; Sreedhar Remya; Harima Meilei; Suzuki Hiroshi; Miyashita Shizuka; Nakamura Takashi; Suzuki Kenji; Nakamura Masahiko; Ueno Kazuyuki; Watanabe Kenichi^*

来源：Epilepsy Research, 2016, 125: 47-51.

DOI：10.1016/j.eplepsyres.2016.06.009

• Fasting
• Hypoglycemic autonomic failure
• Hypoglycemic seizures
• Ketone bodies

Objective: Fasting (48 h) in mice causes resistance to insulin-induced hypoglycemic seizures (IIHS) but in rats fasting (14-16 h) predisposes IIHS. So we suspect the duration of fasting may possibly affect the onset of seizures and in this study, we investigated the IIHS by administering 8 Units (U) insulin (INS)/k.g., intraperitoneally to 8 weeks old male C57BL6/J mice.
Methods: The mice were divided into group 1 (non-fasted), group 2 (6 h fasted) and group 3 (24 h fasted) and we administered the 8 U INS. The first behavioral hypoglycemic seizure symptoms such as jump, clonus or barrel rotations considered as seizure onset and we analyzed the blood glucose level (BGL) and serum beta-hydroxybutyrate (BHB) level.
Results: The time of first seizure onset in group 1 was 109.7 +/- 4.3 min, group 2 was 46.50 +/- 3.9 min and group 3 was 165.4 +/- 13.26 min. The seizure onset time in group 2 was significantly decreased compared to group 1. The seizure onset time in group 3 was significantly increased compared to group 1 and group 2. The decreased BGL after INS administration was correlated with the seizure onset time in group 1 and group 2 but not in group 3. The BHB level in group 3 was significantly higher compared to group 1 and 2.
Conclusion: Our data show that the fasting time duration significantly modulates the onset of hypoglycemic seizures. The opposite effect of 6h or 24 h fasting time duration is likely caused by different BHB levels.

• 出版日期2016-9