Comparative In Vitro Antifungal Susceptibility Activity of Amphotericin B Versus Amphotericin B Methyl Ester Against Candida albicans Ocular Isolates

作者：Thanathanee Onsiri; Miller Darlene; Ringel David M; Schaffner Carl P; Alfonso Eduardo C; O' Brien Terrence P

来源：Journal of Ocular Pharmacology and Therapeutics, 2012, 28(6): 589-592.

DOI：10.1089/jop.2012.0048

Purpose: To compare in vitro susceptibility of amphotericin B (AMB) and amphotericin B methyl ester (AME) (a more soluble and less toxic formulation of AMB) against Candida albicans isolates recovered from human cases of endophthalmitis. %26lt;br%26gt;Methods: The in vitro susceptibility of AMB and AME was determined for C. albicans isolates recovered from endophthalmitis (N = 10) and for C. albicans ATCC reference strain 90028 using the Clinical and Laboratory Standards Institute M27-A2 (NCCLS/CLSI) broth dilution method. All isolates were obtained from samples of vitreous humor of patients with suspected endophthalmitis within the last 5 years at the Bascom Palmer Eye Institute, University of Miami Miller School of Medicine (Miami, FL). %26lt;br%26gt;Results: The minimal inhibitory concentrations (MICs) of AME were equal to or lower than values for AMB in 7 of the 10 isolates; range: AME (0.125-1 mu g/mL) versus (0.5-1 mu g/mL) for AMB. The MIC90 value of both drugs was equal (1 mu g/mL). Compared with AMB, the minimal fungicidal concentrations (MFCs) of AME were equal to or lower in 8 of 10 isolates; range: AME (0.125-2 mu g/mL) versus AMB (0.25-4 mu g/mL). MFC90 values of AME (1 mu g/mL) was slightly superior to AMB (2 mu g/mL). The MIC of the quality control strain (ATCC (R) 90028) was within an acceptable range. %26lt;br%26gt;Conclusions: AME was equivalent to AMB in vitro against C. albicans. This formula may offer a slightly more efficient and less toxic formulation for the treatment of Candida endophthalmitis.

• 出版日期2012-12