A new turn-on fluorescent chemosensor based on diketopyrrolopyrrole (DPP) for imaging Zn2+ in living cells

作者：Zhang, Guanjun; Li, Haiying; Bi, Shiming; Song, Longfeng; Lu, Yunxiang; Zhang, Liang; Yu, Jianjun; Wang, Limin^*

来源：Analyst, 2013, 138(20): 6163-6170.

DOI：10.1039/c3an00873h

• SELECTIVE DETECTION
• ZINC
• PROBE
• FLUORIDE
• ZN(II)
• SENSOR
• ION
• COMPLEX
• PET
• ICT

A new turn-on fluorescent chemosensor, DPPL1, was rationally designed and synthesized based on diketopyrrolopyrrole (DPP), which presented high sensitivity and selectivity for Zn2+. Specifically, DPPL1 presented a large emission enhancement and a 70 nm blue-shift upon Zn2+ binding. H-1 NMR titrations, studies into the pH effects and DFT calculations further proved that this phenomenon was due to both photo-induced electron transfer (PET) and intra-molecular charge transfer (ICT) processes. More importantly, DPPL1 was successfully used for imaging Zn2+ in living HeLa cells. To the best of our knowledge, this is the first DPP-based fluorescent chemosensor to recognize Zn2+ ions in living cells with a large blue-shift and a fluorescent enhancement based on both photo-induced electron-transfer (PET) and intramolecular charge-transfer (ICT) effects. Meanwhile, we have also provided a new effective platform for the development of fluorescent chemosensors for other analytes.

• 出版日期2013
• 单位华东理工大学