Are bacterial load and synovitis related in dogs with inflammatory stifle arthritis?

作者：Schwartz Zeev; Zitzer Nina C; Racette Molly A; Manley Paul A; Schaefer Susan L; Markel Mark D; Hao Zhengling; Holzman Gerianne; Muir Peter^*

来源：Veterinary Microbiology, 2011, 148(2-4): 308-316.

DOI：10.1016/j.vetmic.2010.09.011

• Dog
• Stifle
• Arthritis
• Bacterial load
• 16S rRNA real-time PCR

It has been proposed that small quantities of microbial material within synovial joints may act as a trigger for development of synovitis. We have previously identified an association between intra-articular bacteria and development of inflammatory stifle arthritis and cranial cruciate ligament rupture (CCLR) in dogs, and now wished to quantify bacterial load and markers of synovitis in dogs with and without stifle arthritis and CCLR. Joint tissues were collected from dogs with CCLR (n = 51) and healthy dogs with normal stifles (n = 9). Arthritis was assessed radiographically in CCLR dogs. Bacterial load was assessed using qPCR and broad-ranging 16S rRNA primers. qRT-PCR was used to estimate expression of the T lymphocyte antigen receptor (TCR v beta), CD3 epsilon, tartrate-resistant acid phosphatase (TRAP), IL-4, IL-17, and TNF-alpha genes. Severity of synovitis was assessed histologically. Bacterial load was increased in arthritic stifles, when compared with healthy stifles. Histologic synovitis in arthritic stifles was mononuclear and was significantly correlated with bacterial load (1 of 2 primer sets) (S(R) = 0.49, p < 0.001). In arthritic stifles, expression of TRAP in synovium was increased relative to healthy stifles. Expression of pro-inflammatory genes was not correlated with bacterial load, histologic inflammation, or radiographic arthritis. Translocation of bacterial material to the canine stifle is related to the presence of joint inflammation. The lack of a strong positive correlation suggests that bacterial load is unlikely to be a primary pro-inflammatory factor. However, dysregulation of immune responses within synovial tissues may be dependent upon an environmental microbial trigger.

• 出版日期2011-3-24