A Robust Algorithm for Segmenting and Tracking Clustered Cells in Time-Lapse Fluorescent Microscopy

作者：Tarnawski Wojciech^*; Kurtcuoglu Vartan; Lorek Pawel; Bodych Marcin; Rotter Jan; Muszkieta Monika; Piwowar Lukasz; Poulikakos Dimos; Majkowski Michal; Ferrari Aldo

来源：IEEE Journal of Biomedical and Health Informatics, 2013, 17(4): 862-869.

DOI：10.1109/JBHI.2013.2262233

• Cell segmentation and tracking
• ellipse fitting
• fluorescent microscopy
• multiphase active contours

We present herein a robust algorithm for cell tracking in a sequence of time-lapse 2-D fluorescent microscopy images. Tracking is performed automatically via a multiphase active contours algorithm adapted to the segmentation of clustered nuclei with obscure boundaries. An ellipse fitting method is applied to avoid problems typically associated with clustered, overlapping, or dying cells, and to obtain more accurate segmentation and tracking results. We provide quantitative validation of results obtained with this new algorithm by comparing them to the results obtained from the established CellProfiler, MTrack2 (plugin for Fiji), and LSetCellTracker software.

• 出版日期2013-7