科研之友
微信
新浪微博
Facebook
分享链接
摘要
Two methods for the large scale preparation of uniformly isotope-labeled DNA for NMR studies have been developed, The first method comprises the growth of a suitable plasmid harboring multiple copies of the desired oligonucleotide in a medium based on N-15 and C-13 nutrients. The second method uses a polymerase chain reaction (PCR)-based approach with N-15-and/or C-13-labeled deoxynucleoside triphosphates. The novelty of our PCR strategy over existing ones is that the primer and template are the identical molecule, resulting in an exponential growth in the length of the double strand that contains tandem repeats of the target DNA sequence. This novel PCR approach, which we have termed ESRA for endonuclease-sensitive repeat amplification, is easy to use, results in high fields, and can be accomplished at low costs, The utility of both methods is demonstrated for the preparation of a double-stranded 21-mer uniformly labeled with N-15 and a double-stranded 17-mer DNA uniformly labeled with N-15 and C-13.
- 出版日期1998-1-23
