Mir-34a Is Upregulated during Liver Regeneration in Rats and Is Associated with the Suppression of Hepatocyte Proliferation

作者：Chen, Huan^*; Sun, Yimin; Dong, Ruiqi; Yang, Shengsheng; Pan, Chuanyong; Xiang, Dao; Miao, Mingyong; Jiao, Binghua

来源：PLos One, 2011, 6(5): e20238.

DOI：10.1371/journal.pone.0020238

• TUMOR-SUPPRESSOR
• GROWTH-FACTOR
• DOWN-REGULATION
• CELL LINE
• ACTIVIN-B
• EXPRESSION
• MICRORNAS
• BETA
• HEPATECTOMY
• FOLLISTATIN

Background: MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination.
Methodology/Principal Finding: MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin bB (INHBB) and Met. In BRL3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver.
Conclusion/Significance: MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34amediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation.

• 出版日期2011-5-31
• 单位中国人民解放军第二军医大学