科研之友
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摘要
Expression of a DNA fragment coding for the 76-208 domain of protein molecule of the epidermal growth factor precursor, which contains a sequence of 133 N-terminal amino acid residues, was attained by gene engineering and molecular biology methods. The fusion protein was produced with very low efficacy in E. coli cells. It was purified by metal-affinity chromatography and its specific antitoxic activity evaluated in guinea pigs was at least 10(6) IU/mg protein.
- 出版日期1999-1
