A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis

作者：Pedrolli Danielle Biscaro; Kuehm Christian; Sevin Daniel C; Vockenhuber Michael P; Sauer Uwe; Suess Beatrix; Mack Matthias^*

来源：Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(45): 14054-14059.

DOI：10.1073/pnas.1515024112

• RibR
• FMN riboswitch
• Bacillus subtilis
• flavin mononucleotide
• riboflavin

Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for biosynthesis and/or transport of riboflavin (rib genes). Cytoplasmic riboflavin is rapidly and almost completely converted to FMN by flavokinases. When cytoplasmic levels of FMN are sufficient ("high levels"), FMN binding to FMN riboswitches leads to a reduction of rib gene expression. We report here that the protein RibR counteracts the FMN-induced "turn-off" activities of both FMN riboswitches in Bacillus subtilis, allowing rib gene expression even in the presence of high levels of FMN. The reason for this secondary metabolic control by RibR is to couple sulfur metabolism with riboflavin metabolism.

• 出版日期2015-11-10
• 单位Univ Estadual Paulista