Acute exogenous TSH administration stimulates leptin secretion in vivo

作者：Santini Ferruccio^*; Galli Giulia; Maffei Margherita; Fierabracci Paola; Pelosini Caterina; Marsili Alessandro; Giannetti Monica; Castagna Maria Grazia; Checchi Serenella; Molinaro Eleonora; Piaggi Paolo; Pacini Furio; Elisei Rossella; Vitti Paolo; Pinchera Aldo

来源：European Journal of Endocrinology, 2010, 163(1): 63-67.

DOI：10.1530/EJE-10-0138

TSH-receptor (TSHR) has been found in a variety of cell types, including preadipocytes and adipocytes. In vitro, TSH-mediated preadipocyte and adipocyte responses include proliferation, differentiation, survival, and lipolysis.
Objective: To measure the response of serum leptin to exogenous administration of recombinant human TSH (rhTSH) in vivo.
Patients: One hundred patients with differentiated thyroid cancer already treated by total thyroidectomy and (131)I remnant ablation were enrolled. Mean (+/-S.E.M.) body mass index (BMI) was 26.9+/-0.6 kg/m(2).
Methods: Patients received a standard dose of rhTSH for measurement of thyroglobulin in the follow-up of their disease. Blood samples were taken for the assay of TSH and leptin before the first administration of rhTSH (time 0), and 24 h (time 1), 48 h (time 2), 72 h (time 3), and 96 h (time 4) after the first administration of rhTSH.
Results: Significant mean serum leptin increments, with respect to basal value, were 16, 13, 18, and 11% at times 1, 2, 3, and 4 respectively. Significant positive correlations of leptin-area under the curve with respect to basal leptin levels (r=0.43; P<0.0001) and BMI (r=0.32; P<0.005) were observed.
Conclusions: Acute rhTSH administration in hypothyroid subjects under L-thyroxine therapy produces a rise in serum leptin. This increase is proportional to the adipose mass suggesting that a functioning TSHR is expressed on the surface of adipocytes. The role that TSHR activation in adipocytes might play in physiological and pathological conditions remains a matter of investigation.

• 出版日期2010-7