A novel approach based on capillary electrophoresis coupled to augmented multivariate curve resolution-alternating least-squares modeling for the determination of pKa of 2-hydroxy-4,6-dimethylpyrimidine in nicarbazin

作者：Teglia Carla M; Brasca Romina; Vera Candioti Luciana; Goicoechea Hector C

来源：Chemometrics and Intelligent Laboratory Systems, 2016, 150: 1-8.

DOI：10.1016/j.chemolab.2015.11.001

• 2-hydroxy-4,6-dimethylpyrimidine
• Nicarbazin
• Capillary electrophoresis
• Augmented multivariate curve resolution
• Second-order data

The determination of the dissociation constant of 2-hydroxy-4,6-dimethylpyrimidine in nicarbazin was carried out by means of capillary electrophoresis using a dynamic coating of a fused-silica capillary with high-molecular-mass poly(diallyldimethylammonium chloride) (PDDA) implementing two methods: (a) fitting the effective mobility determined from different electrophoretic runs at a single wavelength as a function of pH and (b) a novel modeling strategy of the spectrophotometric second-order data generated with the diode array detector at several wavelengths as a function of pH. The accuracy of the proposed methodologies was verified using robenidine as a model compound with known value of pKa.

• 出版日期2016-1-15