Building the Connectivity Map of epigenetics: Chromatin profiling by quantitative targeted mass spectrometry

作者：Creech Amanda L; Taylor Jordan E; Maier Verena K; Wu Xiaoyun; Feeney Caitlin M; Udeshi Namrata D; Peach Sally E; Boehm Jesse S; Lee Jeannie T; Carr Steven A; Jaffe Jacob D^*

来源：Methods, 2015, 72: 57-64.

DOI：10.1016/j.ymeth.2014.10.033

• Histones
• Chromatin
• Epigenetics
• Proteomics
• Mass spectrometry
• SILAC

Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.

• 出版日期2015-1-15
• 单位MIT