An optimized two-finger archive for ZFN-mediated gene targeting

作者：Gupta Ankit; Christensen Ryan G; Rayla Amy L; Lakshmanan Abirami; Stormo Gary D; Wolfe Scot A^*

来源：Nature Methods, 2012, 9(6): 588-+.

DOI：10.1038/NMETH.1994

The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.

• 出版日期2012-6