Application of LW7 marker for identification of progenies with male sterility gene in sweet sorghum population

作者：Bunphan Darika^*; Jaisil Prasit; Sanitchon Jirawat

来源：Crop Breeding and Applied Biotechnology, 2013, 13(1): 59-66.

• Cytoplasmic genetic male sterile
• restorer gene
• energy crop
• marker assisted selection

The objectives of this study were to verify the use of LW7 marker in identifying maintainer lines (B-lines) and restorer lines (R-lines) in grain sorghum and sweet sorghum, and to identify B-lines in the F-2, BC1F2 and BC2F2 generations. Twenty five accessions of sorghum were evaluated, and LW7 marker correctly identified accessions which presented male sterility gene (rf4) in Suphan Buri1 and 03B cultivars; moreover, these genotypes did not show 779 bp band. The cross between Suphan Buri1 and a male-sterile line (A-line) 03A resulted in a sterilized male, confirming the usefulness of the marker in breeding programs. B-lines in the F-2, BC1F2 and BC2F2 generations were identified by LW7 marker. The segregation ratio of 3:1 for male fertility and male sterility in the progenies of the three generations supported the one-gene model of Mendelian segregation. The use of marker assisted selection was successful for line development of sweet sorghum with male sterility.

• 出版日期2013-3