A novel piezoelectric immunosensor for the detection of malarial Plasmodium falciparum histidine rich protein-2 antigen

作者：Sharma M K; Rao V K^*; Merwyn S; Agarwal G S; Upadhyay Sanjay; Vijayaraghavan R

来源：Talanta, 2011, 85(4): 1812-1817.

DOI：10.1016/j.talanta.2011.07.008

• PfHRP-2 antigen
• Malaria
• Piezoelectric quartz crystal
• SAMs
• Immunosensor

A novel piezoelectric (PZ) immunosensor for the direct detection of malarial Plasmodium falciparum histidine rich protein-2 (PfHRP-2) antigen was developed. The mixed self-assembled monolayers (SAMs) of thioctic acid and 1-dodecanethiol were formed on gold surface of quartz crystal. Cyclic voltammetry, electrochemical impedance spectroscopy and surface Raman spectroscopy techniques were used to characterize the mixed SAMs. The rabbit anti-PfHRP-2 antibodies were coupled on mixed SAM modified gold surface of quartz crystal via NHS/EDC activation method. The PZ immunosensor was applied to detect PfHRP-2 in the linear range of 15-60 ng/ml with a detection limit of 12 ng/ml. It was also found that even after 14 days of storage, 50% of the activity still remained. Clinical human serum samples were tested with this method, and the results were in agreement with those obtained from commercially available ICT kit (NOW (R) Malaria).

• 出版日期2011-9-30