alpha-Actinin4 nuclear translocation mediates gonadotropin-releasing hormone stimulation of follicle-stimulating hormone beta-subunit gene transcription in L beta T2 cells

作者：Yu Han; Li Zhengjun; Ghosh Dipanjana; Lim Teck Kwang; He Yuehui; Lin Qingsong^*

来源：FEBS LETTERS, 2012, 586(10): 1466-1471.

DOI：10.1016/j.febslet.2012.03.067

• GnRH
• Fsh beta
• ACTN4
• Calmodulin-like domain
• Nuclear translocation

Gonadotropin-releasing hormone (GnRH) regulates the synthesis and secretion of follicle-stimulating hormone (FSH) by stimulating the transcription of Fsh beta gene. Our iTRAQ quantitative proteomics result showed that the abundance of alpha-actinin4 (ACTN4) increased in the nuclei of L beta T2 cells upon GnRH induction. Using RNA interference, reverse transcription and real-time PCR, luciferase and transient transfection assays, we proved that ACTN4 is involved in the regulation of mouse Fsh beta gene (mFsh beta) transcription and its C-terminal calmodulin (CaM)-like domain is crucial for this process. Our study suggests that ACTN4 nuclear translocation mediates GnRH stimulation of mFsh beta gene transcription.

• 出版日期2012-5-21