AMPA Receptors Regulate Exocytosis and Insulin Release in Pancreatic beta Cells

作者：Wu, Zhen-Yong; Zhu, Li-Jun; Zou, Na; Bombek, Lidija K.; Shao, Chong-Yu; Wang, Na; Wang, Xin-Xin; Liang, Li; Xia, Jun; Rupnik, Marjan; Shen, Ying^*

来源：Traffic, 2012, 13(8): 1124-1139.

DOI：10.1111/j.1600-0854.2012.01373.x

• ss cell
• AMPA receptor
• cGMP
• exocytosis
• insulin
• KATP channel

Ionotropic glutamate receptors (iGluRs) are expressed in islets and insulinoma cells and involved in insulin secretion. However, the exact roles that iGluRs play in beta cells remain unclear. Here, we demonstrated that GluR2-containing a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) were expressed in mouse beta cells. Glutamate application increased both cytosolic calcium and the number of docked insulin-containing granules, which resulted in augmentation of depolarization-induced exocytosis and high-glucose-stimulated insulin release. While glutamate application directly depolarized beta cells, it also induced an enormous depolarization when KATP channels were available. Glutamate application reduced the conductance of KATP channels and increased voltage oscillations. Moreover, actions of AMPARs were absent in Kir6.2 knock-out mice. The effects of AMPARs on KATP channels were mediated by cytosolic cGMP. Taken together, our experiments uncovered a novel mechanism by which AMPARs participate in insulin release.

• 出版日期2012-8
• 单位香港科技大学; 浙江大学