Age-Associated Proinflammatory Secretory Phenotype in Vascular Smooth Muscle Cells From the Non-human Primate Macaca mulatta: Reversal by Resveratrol Treatment

作者：Csiszar Anna; Sosnowska Danuta; Wang Mingyi; Lakatta Edward G; Sonntag William E; Ungvari Zoltan^*

来源：Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 2012, 67(8): 811-820.

DOI：10.1093/gerona/glr228

• Vascular aging
• Inflammation
• Oxidative stress
• Cytokine-3,5,4 %26apos
• -trihydroxy-trans-stilbene

There is increasing evidence that age-associated chronic low-grade inflammation promotes the development of both large-vessel disease (myocardial infarction, stroke, peripheral arterial disease) and small-vessel pathologies (including vascular cognitive impairment) in older persons. However, the source of age-related chronic vascular inflammation remains unclear. To test the hypothesis that cell-autonomous mechanisms contribute to the proinflammatory changes in vascular phenotype that accompanies advancing age, we analyzed the cytokine secretion profile of primary vascular smooth muscle cells (VSMCs) derived from young (similar to 13 years old) and aged (similar to 21 years old) Macaca mulatto. Aged VSMCs cultured in the absence of systemic factors exhibited significantly increased secretion of interleukin-1 beta, MCP-1, and tumor necrosis factor alpha compared with young control cells. Secretion of interleukin-6 also tended to increase in aged VSMCs. This age-associated proinflammatory shift in the cellular secretory phenotype was associated with an increased mitochondrial O-2(-) production and nuclear factor kappa-light-chain-enhancer of activated B cells activation. Treatment of aged VSMCs with a physiologically relevant concentration of resveratrol (1 mu M) exerted significant anti-inflammatory effects, reversing aging-induced alterations in the cellular cytokine secretion profile and inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells. Resveratrol also attenuated mitochondrial O-2(-) production and upregulated the transcriptional activity of Nrf2 in aged VSMCs. Thus, in non-human primates, cell-autonomous activation of nuclear factor kappa-light-chain-enhancer of activated B cells and expression of an inflammatory secretome likely contribute to vascular inflammation in aging. Resveratrol treatment prevents the proinflammatory properties of the aged VSMC secretome, an effect that likely contributes to the demonstrated vasoprotective action of resveratrol in animal models of aging.

• 出版日期2012-8