Antigenic structures stably expressed by recombinant TGEV-derived vectors

作者：Becares Martina; Sanchez Carlos M; Sola Isabel; Enjuanes Luis^*; Zuniga Sonia

来源：Virology, 2014, 464: 274-286.

DOI：10.1016/j.virol.2014.07.027

• Positive-strand RNA viruses
• RNA vector stability
• Coronavirus derived vectors
• TGEV
• PRRSV

Coronaviruses (CoVs) are positive-stranded RNA viruses with potential as immunization vectors, expressing high levels of heterologous genes and eliciting both secretory and systemic immune responses. Nevertheless, its high recombination rate may result in the loss of the full-length foreign gene, limiting their use as vectors. Transmissible gastroenteritis virus (TGEV) was engineered to express porcine reproductive and respiratory syndrome virus (PRRSV) small protein domains, as a strategy to improve heterologous gene stability. After serial passage in tissue cultures, stable expression of small PRRSV protein antigenic domains was achieved. Therefore, size reduction of the heterologous genes inserted in CoV-derived vectors led to the stable expression of antigenic domains. Immunization of piglets with these TGEV vectors led to partial protection against a challenge with a virulent PRRSV strain, as immunized animals showed reduced clinical signs and lung damage. Further improvement of TGEV-derived vectors will require the engineering of vectors with decreased recombination rate.

• 出版日期2014-9