A novel near-infrared probe for the imaging of superoxide anion fluctuations and hydrogen ion enhancement in vivo

作者：Sun, Chunlong^*; Du, Wen^*; Zhang, Wei; Wu, Yang; Yao, Zhigang; Wang, Baoqin; Wu, Tao; Yang, Hongjun; Wang, YanMei; Ren, Lili

来源：Analytical Methods, 2018, 10(30): 3727-3736.

DOI：10.1039/c8ay01190g

• 2-PHOTON FLUORESCENT-PROBE
• MINOR PH CHANGES
• LIVING CELLS
• ISCHEMIA-REPERFUSION
• OXIDATIVE STRESS
• MITOCHONDRIAL PH
• INTRACELLULAR PH
• TUMOR-DIAGNOSIS
• OXYGEN RADICALS
• LIVE CELLS

Superoxide anions (O-2(-)) and hydrogen ions (H+) have attracted considerable attention in studies on cellular dysfunction. A newly synthesized reaction-based near-infrared imaging probe (IR-747-SAPH), which is composed of a superoxide anion responsive group and a H+ responsive group, exhibits high selectivity to O-2(-) and H+ fluxes in vivo. The fluorescence intensity of IR-747-SAPH changes as O-2(-) and H+ concentrations vary. IR-747-SAPH is characterized by sensitivity to changes in H+, high photostability, and strong near-infrared properties, including high temporal resolution, deep penetration, and low background interference. The probe also provides reliable near-infrared fluorescence signals that alter O(2)(-)and H+ levels in vivo. Therefore, IR-747-SAPH fluorescence imaging is a promising detection method for monitoring the changes in O-2(-) and H+ in various biological environments.

• 出版日期2018-8-14
• 单位南京医科大学; 江苏省肿瘤防治研究所; 滨州学院