Measuring kinetic rate constants of multiple-component reactions with optical biosensors

作者：Edwards David A; Evans Ryan M^*; Li Wenbin

来源：Analytical Biochemistry, 2017, 533: 41-47.

DOI：10.1016/j.ab.2017.06.009

• Optical biosensors
• Kinetic rate constants
• Mathematical modeling
• Parameter identification

One may measure the kinetic rate constants associated with biochemical reactions using an optical biosensor: an instrument in which ligand molecules are convected. through a flow cell over a surface to which receptors are immobilized. If there are multiple reactants, one is faced with the problem of fitting multiple kinetic rate constants to one signal, since data from all of the reacting species is lumped together. Even in the presence of ambiguous data, one may use a series of experiments to accurately determine the rate constants. Moreover, the true set of rate constants may be identified by either postprocessing the signals or adjusting the ligand inflow concentrations. Published by Elsevier Inc.

• 出版日期2017-9-15