A highly sensitive signal-amplified gold nanoparticle-based electrochemical immunosensor for dibutyl phthalate detection

作者：Liang, Ya-Ru; Zhang, Zong-Mian^*; Liu, Zhen-Jiang; Wang, Kun; Wu, Xiang-Yang; Zeng, Kun; Meng, Hui; Zhang, Zhen

来源：Biosensors and Bioelectronics, 2017, 91: 199-202.

DOI：10.1016/j.bios.2016.12.007

• Electrochemical Immunosensor
• Signal Amplification
• Antibody
• Gold Nanoparticles
• Dibutyl Phthalate

A simple and signal-amplified, label-free electrochemical impedimetric immunosensor for dibutyl phthalate (DBP), a type of phthalate ester, was developed using gold nanoparticles (AuNPs) induced signal amplification. The approach was based on an indirectly competitive binding system of coating antigen that coated on antigen/chitosan/MWCNTs@GONRs/GCE (modified electrodes), target (DBP) and anti-DBP antibody. Signal amplification was performed via the enlargement of AuNPs through NADH-promoted catalytic precipitation, which caused a marked increase in the electron-transfer resistance and electrostatic repulsion after an AuNP-labeled second antibody conjugated to anti-DBP. Under the optimized conditions, this method displayed: (i) low detection limits (7 ng/mL, 10 times lower than the traditional ELISA method using the same antibody); (ii) satisfactory accuracy (recoveries, 86.0-120.4%) and agreement with corresponding ELISA method; (iii) high tolerance to some environmental interferents; and (iv) low cost and low sample consumption (6 L). Our results demonstrate the great potential and high efficiency of this immunosensor in practical applications for the costeffective and sensitive monitoring of DBP in aquatic environments.

• 出版日期2017-5-15
• 单位江苏大学