Amyloid-beta 42 adsorption following serial tube transfer

作者：Toombs Jamie^*; Paterson Ross W; Schott Jonathan M; Zetterberg Henrik

来源：Alzheimer's Research and Therapy, 2014, 6(1): 5.

DOI：10.1186/alzrt236

Introduction: Cerebrospinal fluid (CSF) amyloid-beta 38 (A beta 38), 40 (A beta 40), 42 (A beta 42) and total tau (T-tau) are finding increasing utility as biomarkers of Alzheimer's disease (AD). The purpose of this study was to determine whether measured CSF biomarker concentrations were affected by transfer of CSF between tubes, and whether addition of a non-ionic surfactant mitigates any observed effects.
Methods: AD and control CSF was transferred consecutively between polypropylene tubes. A beta peptides and T-tau were measured with and without addition of Tween 20 (0.05%).
Results: Measured concentrations of A beta 42 decreased by approximately 25% with each consecutive transfer. Measured concentrations of A beta 38 and A beta 40 were also observed to decrease significantly with each consecutive transfer (approximately 16% loss per transfer). Measured concentrations of T-tau also decreased significantly, but at much smaller magnitude than the A beta peptides (approximately 4% loss per transfer). The addition of Tween 20 mitigated this effect in all samples.
Conclusions: Consecutive CSF transfer between tubes has a significant impact on the measured concentration of all A beta peptides, and significant effect of lesser magnitude on T-tau. This would be sufficient to alter biomarker ratios enough to mislead diagnosis. The introduction of Tween 20 at the initial aliquoting stage was observed to significantly mitigate this effect.

• 出版日期2014