A conserved Toll-like receptor-to-NF-kappa B signaling pathway in the endangered coral Orbicella faveolata

作者：Williams Leah M; Fuess Lauren E; Brennan Joseph J; Mansfield Katelyn M; Salas Rodriguez Erick; Welsh Julianne; Awtry Jake; Banic Sarah; Chacko Cecilia; Chezian Aarthia; Dowers Donovan; Estrada Felicia; Hsieh Yu Hsuan; Kang Jiawen; Li Wanwen; Malchiodi Zoe; Malinowski John; Matuszak Sean; McTigue Thomas; Mueller David; Nguyen Brian; Nguyen Michelle; Phuong Nguyen; Nguyen Sinead; Njoku Ndidi; Patel Khu**u; Pellegrini William; Pliakas Tessa; Qadir Deena; Ryan Emma

来源：Developmental and Comparative Immunology, 2018, 79: 128-136.

DOI：10.1016/j.dci.2017.10.016

• NF-kappaB
• Toll-like receptor
• Evolution
• Coral
• Innate immunity
• Orbicella faveolata

Herein, we characterize the Toll-like receptor (TLR)-to-NF-kappa B innate immune pathway of Orbicella faveolata (Of), which is an ecologically important, disease-susceptible, reef-building coral. As compared to human TLRs, the intracellular TIR domain of Of-TLR is most similar to TLR4, and it can interact in vitro with the human TLR4 adapter MYD88. Treatment of O. faveolata tissue with lipopolysaccharide, a ligand for mammalian TLR4, resulted in gene expression changes consistent with NF-kappa B pathway mobilization. Biochemical and cell-based assays revealed that Of-NB-kappa B resembles the mammalian non-canonical NF-kappa B protein p100 in that C-terminal truncation results in translocation of Of-NF-kappa B to the nucleus and increases its DNA-binding and transcriptional activation activities. Moreover, human I kappa B kinase (IKK) and Of-IKK can both phosphorylate conserved residues in Of-NF-kappa B in vitro and induce C-terminal processing of Of-NF-kappa B in vivo. These results are the first characterization of TLR-to-NF-kappa B signaling proteins in an endangered coral, and suggest that these corals have conserved innate immune pathways.

• 出版日期2018-2