Artificial enzymes based on cyclodextrin with phenol as the catalytic group

作者：Lindback Emil; Zhou You; Pedersen Christian M^*; Bols Mikael

来源：Tetrahedron Letters, 2012, 53(37): 5023-5026.

DOI：10.1016/j.tetlet.2012.07.050

• Chemzymes
• Glycosidase
• Cyclodextrin
• Catalysis

beta-Cyclodextrin containing one or two N-linked ortho-N-acetylaminophenols has been synthesized and tested for their properties as artificial glycosidases. Four different para-nitrophenyl glycosides were used as substrates and k(cat)/k(uncat) values of up to 381 were found for the di-functionalized products, whereas the mono derivative showed up to 47 times rate enhancement. The optimum pH for the catalysis was found to be approximately pH 8 and the influence of phosphate concentration was investigated.

• 出版日期2012-9-12