An aptamer-based chromatographic strip assay for sensitive toxin semi-quantitative detection

作者：Wang, Libing; Ma, Wenwei; Chen, Wei; Liu, Liqiang; Ma, Wei; Zhu, Yingyue; Xu, Liguang; Kuang, Hua^*; Xu, Chuanlai

来源：Biosensors and Bioelectronics, 2011, 26(6): 3059-3062.

DOI：10.1016/j.bios.2010.11.040

• Aptamer
• Gold nanoparticle
• Strip
• Ochratoxin A

An aptamer-based chromatographic strip assay method for rapid toxin detection was developed. The aptamer-based strip assay was based on the competition for the aptamer between ochratoxin A and DNA probes. The sensing results indicated that the sensitivity of the aptamer-based strip was better than that of conventional antibody-based strips. The visual limit of detection of the strip for qualitative detection was 1 ng/mL while the LOD for semi-quantitative detection could down to 0.18 ng/mL by using scanning reader. The recoveries of test samples were from 96% to 110%. All detections could be achieved in less than 10 min, indicating that the aptamer-based strip could be a potential useful tool for rapid on-site detections.

• 出版日期2011-2-15
• 单位食品科学与技术国家重点实验室; 江南大学