Adenosine receptors regulate susceptibility to noise-induced neural injury in the mouse cochlea and hearing loss

作者：Vlajkovic Srdjan M; Ambepitiya Kaushi; Barclay Meagan; Boison Detlev; Housley Gary D; Thorne Peter R

来源：Hearing Research, 2017, 345: 43-51.

DOI：10.1016/j.heares.2016.12.015

• Adenosine receptors
• Noise
• Hearing loss
• Cochlea
• Spiral ganglion neurons
• Synaptic ribbons

Our previous studies have shown that the stimulation of Al adenosine receptors in the inner ear can mitigate the loss of sensory hair cells and hearing loss caused by exposure to traumatic noise. Here, we focus on the role of adenosine receptors (AR) in the development of noise-induced neural injury in the cochlea using A(1)AR and A(2A)AR null mice (A(1)AR(-/-) and A(2A)AR(-1-)). Wildtype (WT) and AR deficient mice were exposed to octave band noise (8-16 kHz, 100 dB SPL) for 2 h to induce cochlear injury and hearing loss. Auditory thresholds and input/output functions were assessed using auditory brainstem responses (ABR) before and two weeks post-exposure. The loss of outer hair cells (OHC), afferent synapses and spiral ganglion neurons (SGN) were assessed by quantitative histology. A(1)AR(-/-) mice (6-8 weeks old) displayed a high frequency hearing loss (ABR threshold shift and reduced ABR wave I and II amplitudes). This hearing loss was further aggravated by acute noise exposure and exceeded the hearing loss in the WT and A(2A)AR(-1-) mice. All mice experienced the loss of OHC, synaptic ribbons and SGN after noise exposure, but the loss of SGN was significantly higher in A(1)AR(-/-) mice than in the A(2A)AR(-1-) and WT genotypes. The A(2A)AR(-1-) demonstrated better preservation of OHC and afferent synapses and the minimal loss of SGN after noise exposure. The findings suggest that the loss of illAR expression results in an increased susceptibility to cochlear neural injury and hearing loss, whilst absence of A2AAR increases cochlear resistance to acoustic trauma.

• 出版日期2017-3