mScarlet: a bright monomeric red fluorescent protein for cellular imaging

作者：Bindels, Daphne S.; Haarbosch, Lindsay; van Weeren, Laura; Postma, Marten; Wieser, Katrin E.; Mastop, Marieke; Aumonier, Sylvain; Gotthard, Guillaume; Royant, Antoine; Hink, Mark A.; Gadella, Theodorus W. J., Jr.^*

来源：Nature Methods, 2017, 14(1): 53-56.

DOI：10.1038/NMETH.4074

• GREEN
• BIOSENSORS
• LIFETIME
• TISSUES
• ORANGE
• CELLS

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Forster resonance energy transfer (FRET) acceptor in ratiometric imaging.

• 出版日期2017-1
• 单位中国地震局