Novel optimized biofunctional surfaces for Love mode surface acoustic wave based immunosensors

作者：Dinh Duy Hai; Pascal Emilie; Vellutini Luc; Bennetau Bernard; Rebiere Dominique; Dejous Corinne; Moynet Daniel; Belin Colette; Pillot Jean Paul^*

来源：Sensors and Actuators B: Chemical , 2010, 146(1): 289-296.

DOI：10.1016/j.snb.2010.02.008

• SAW immunosensor
• Biofunctionalization
• Antibodies
• Very-long-chain glycidyl-terminated SAM
• Surface smoothing
• E. coli

A very-long-chain (C-22) organosilicon self-assembled monolayer (SAM) with terminal glycidyl groups was used to immobilize antibodies on the silica waveguide of a Love mode surface acoustic wave (SAW) immunosensor. This route enabled us to specifically detect, in real time, living Escherichia coli bacteria by using monoclonal anti-E. coli antibodies, leading to a marked sensor response in liquid aqueous medium. Conversely, no bacteria detection could be observed by this direct method upon functionalization of the waveguide surface by a (3-glycidoxypropyl) trimethoxysilane (GPIS) monolayer. This improvement was attributed to a surface smoothing effect resulting from the very-long-chain SAM and a better accessibility of the surface epoxide groups.

• 出版日期2010-4-8