An Elevation in Physical Coupling of Type 1 Inositol 1,4,5-Trisphosphate (IP3) Receptors to Transient Receptor Potential 3 (TRPC3) Channels Constricts Mesenteric Arteries in Genetic Hypertension

作者：Adebiyi Adebowale; Thomas Gatewood Candice M; Leo M Dennis; Kidd Michael W; Neeb Zachary P; Jaggar Jonathan H^*

来源：Hypertension, 2012, 60(5): 1213-+.

DOI：10.1161/HYPERTENSIONAHA.112.198820

• hypertension
• vasoconstriction
• canonical transient receptor potential channels
• inositol 1,4,5-trisphosphate receptors
• endothelin-1

Hypertension is associated with an elevation in agonist-induced vasoconstriction, but mechanisms involved require further investigation. Many vasoconstrictors bind to phospholipase C-coupled receptors, leading to an elevation in inositol 1,4,5-trisphosphate (IP3) that activates sarcoplasmic reticulum IP3 receptors. In cerebral artery myocytes, IP3 receptors release sarcoplasmic reticulum Ca2+ and can physically couple to canonical transient receptor potential 3 (TRPC3) channels in a caveolin-1-containing macromolecular complex, leading to cation current activation that stimulates vasoconstriction. Here, we investigated mechanisms by which IP3 receptors control vascular contractility in systemic arteries and IP3R involvement in elevated agonist-induced vasoconstriction during hypertension. Total and plasma membrane-localized TRPC3 protein was approximate to 2.7- and 2-fold higher in mesenteric arteries of spontaneously hypertensive rats (SHRs) than in Wistar-Kyoto (WKY) rat controls, respectively. In contrast, IP(3)R1, TRPC1, TRPC6, and caveolin-1 expression was similar. TRPC3 expression was also similar in arteries of pre-SHRs and WKY rats. Control, IP3-induced and endothelin-1 (ET-1)-induced fluorescence resonance energy transfer between IP(3)R1 and TRPC3 was higher in SHR than WKY myocytes. IP3-induced cation current was approximate to 3-fold larger in SHR myocytes. Pyr3, a selective TRPC3 channel blocker, and calmodulin and IP3 receptor binding domain peptide, an IP3R-TRP physical coupling inhibitor, reduced IP 3 induced cation current and ET-1-induced vasoconstriction more in SHR than WKY myocytes and arteries. Thapsigargin, a sarcoplasmic reticulum Ca2+-ATPase blocker, did not alter ET-1-stimulated vasoconstriction in SHR or WKY arteries. These data indicate that ET-1 stimulates physical coupling of IP(3)R1 to TRPC3 channels in mesenteric artery myocytes, leading to vasoconstriction. Furthermore, an elevation in IP(3)R1 to TRPC3 channel molecular coupling augments ET-1-induced vasoconstriction during hypertension. (Hypertension. 2012;60:1213-1219.). Online Data Supplement

• 出版日期2012-11