科研之友
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摘要
Despite the presence of Zn2+ in high levels in Parkinson brain, it is not yet clearly answered whether and how Zn2+ alters the electrical activity of neurons in substantia nigra (SN). Here we show that Zn2+ alters the intrinsic activity of nigral dopamine neurons in dual ways, that is, excitation or inhibition, by modulating the gating properties of a transient A-type K+ (K-A) channel. Depending on the holding potential, Zn2+ could reduce or enhance a transient outward K+ current (I-A) in nigral dopamine neurons. Zn2+ slowed the kinetics of both I-A activation and inactivation with the rate of activation much more reduced than that of inactivation. Zn2+ also increased the rate of release from I-A inactivation. Both activation and inactivation I-A curves were shifted by Zn2+ towards positive potentials, but the positive shift of the inactivation curve was much greater than that of the activation curve. We propose that all these effects of Zn2+ on K-A channel gating properties underlie the dual mode of Zn2+ action on I-A, that is, attenuation or potentiation depending on membrane potential. As a result, Zn2+ increased a bursting activity of a nigral dopamine neuron elicited by anodal break excitation presumably through I-A reduction at a hyperpolarizing state, whereas Zn2+ decreased its tonic activity at either resting or depolarizing states where I-A was increased. This was further supported by the observations that 4-aminopyridine (4-AP), a well-known K-A channel blocker, strengthened or counteracted the effect of Zn2+ on the intrinsic excitability of nigral dopamine neurons.
- 出版日期2011-2-17
