Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein

作者：Wu CX; Zhao WP; Kishi H^*; Dokan J; Jin ZX; Wei XC; Yokoyama KK; Muraguchi A

来源：Biochemical and Biophysical Research Communications, 2004, 317(4): 1096-1102.

DOI：10.1016/j.bbrc.2004.03.159

• RAG-2
• promoter
• MAZ
• Pax-5
• c-Myb
• LEF-1

Recombination activating gene-1 (RAG-1) and RAG-2 are expressed specifically in lymphocytes undergoing the antigen receptor gene rearrangement during the lymphocyte development. Our previous study showed that the -41 to -17 nucleotides (nt) 5'-up-stream region of mouse RAG-2 were pre-requisite for the core promoter activity and that Pax-5/c-Myb/LEF-1 protein-protein complex was responsible for its activity in immature B cells. In this study, we show that the -65/-42 sequence, the non-conserved sequence between human and mouse RAG-2 promoter, is necessary for the full promoter activity for mouse RAG-2. Electrophoresis mobility shift assay revealed that Myc-associated zinc finger protein (MAZ) as well as SPI/3 binds a GA box in this region. Using chromatin immunoprecipitation, we show that MAZ binds the RAG-2 promoter region in pre-B cells. Furthermore, we show that MAZ synergistically activates the murine RAG-2 promoter with Pax-5/c-Myb/LEF-1 complex. These results first demonstrate that MAZ participates in activation of mouse RAG-2 promoter.

• 出版日期2004-5-14
• 单位北京大学