Acadl-SNP based genotyping assay for long-chain acyl-CoA dehydrogenase deficient mice

作者：Luther Rita J; Almodovar Alvin J O; Fullerton Russell; Wood Philip A^*

来源：Molecular Genetics and Metabolism, 2012, 106(1): 62-67.

DOI：10.1016/j.ymgme.2012.02.009

• Mouse model
• Long-chain acyl-CoA dehydrogenase
• Acadl alleles
• SNP genotyping assay
• Molecular genotyping

The long-chain acyl-CoA dehydrogenase (LCAD) (Acadl=gene; LCAD=protein) deficient mouse model has been important in evaluating the role of mitochondrial fatty acid oxidation of long-chain fatty acids in metabolic disorders. The insertion vector-based gene targeting strategy used to generate this model has made it difficult to distinguish homozygous and heterozygous genotypes containing targeted Acadl alleles in LCAD-deficient mice. Herein, we describe the design and validation of Acadl SNP genotyping methods capable of distinguishing between heterozygous and homozygous LCAD-deficient mice. The Acadl SNP genotyping assays are effective at allelic discrimination of both C57BL/6 and 129 mouse strain-based Acadl alleles under conditions including, both low purity and quantity genomic DNA templates. This makes the method practical and provides the necessary tools for genotyping the LCAD-deficient mouse model.

• 出版日期2012-5