A new method for enzymatic synthesis of xylose diester in two-solvent medium and its structural identification

作者：Feng, Guang-Li; Li, Xiao-Feng; Zhang, Ben; Zhao, Guang-Lei

来源：Modern Food Science and Technology, 2013, 29(6): 1222-1226.

• Amphoteric compounds
• Biocatalysis
• Environmentally-friendly
• Enzymatic synthesis
• Immobilized lipase
• Reaction temperature
• Structural identification
• Substrate conversion

A new method for synthesis of xylose esters based on solvent strategy was developed in this paper. Binary organic solvent of n-hexane and pyridine (25:75, V/V) was used as reaction system to well dissolve sugar substrate and meanwhile maintain the enzyme activity. Immobilized lipase CAL type B, Novozyme 435, was chosen as a biocatalyst to synthesize xylose ester via acylation of xylose and vinyl methacrylate. HPLC analysis showed that the highest substrate conversion (>90%) was obtained when the ratio of xylose to vinyl methacrylate, shaking speed and reaction temperature were 1:10, 150 r/min and 30°C, respectively. The products were structurally identified by GC-MS and 13C-NMR as a diester of xylose, 1', 5'-dimethacrylic xylose ester. This research provided an efficient and environmentally-friendly method with application potential in production of amphoteric compounds used in food, chemical and papermaking industrils.

• 出版日期2013-6
• 单位华南理工大学