Antitumor Activity of HM781-36B, Alone or in Combination with Chemotherapeutic Agents, in Colorectal Cancer Cells

作者：Kang Mi Hyun; Moon Sung Ung; Sung Ji Hea; Kim Jin Won; Lee Keun Wook; Lee Hye Seung; Lee Jong Seok; Kim Jee Hyun^*

来源：Cancer Research and Treatment, 2016, 48(1): 355-364.

DOI：10.4143/crt.2014.260

• HM781-36B
• Colorectal neoplasms
• Epidermal growth factor receptor-neu receptor
• BMX

Purpose
HM781-36B is a novel and irreversible pan-human epidermal growth factor receptor (HER) inhibitor with TEC cytoplasmic kinase inhibition. The aim of this study is to evaluate the antitumor activity and mechanism of action for HM781-36B in colorectal cancer (CRC) cell lines.
Materials and Methods
The CRC cell lines were exposed to HM781-36B and/or oxaliplatin (L-OHP), 5-fluorouracil (5-FU), SN-38. The cell viability was examined by Cell Titer-Glo luminescent cell viability assay kit. Change in the cell cycle and protein expression was determined by flow cytometry and immunoblot analysis, respectively. Synergism between two drugs was evaluated by the combination index.
Results
The addition of HM781-36B induced potent growth inhibition in both DiFi cells with epidermal growth factor receptor (EGFR) overexpression and SNU-175 cells (IC50, 0.003 mu M and 0.005 mu M, respectively). Furthermore, HM781-36B induced G1 arrest of the cell cycle and apoptosis, and reduced the levels of HER family and downstream signaling molecules, pERK and pAKT, as well as nonreceptor/cytoplasmic tyrosine kinase, BMX. The combination of HM781-36B with 5-FU, L-OHP, or SN-38 showed an additive or synergistic effect in most CRC cells.
Conclusion
These findings suggest the potential roles of HM781-36B as the treatment for EGFR-over-expressing colon cancer, singly or in combination with chemotherapeutic agents. The role of BMX expression as a marker of response to HM781-36B should be further explored.

• 出版日期2016-1