Aligned natural-synthetic polyblend nanofibers for peripheral nerve regeneration

作者：Wang, Chun-Yang; Zhang, Kui-Hua; Fan, Cun-Yi^*; Mo, Xiu-Mei; Ruan, Hong-Jiang; Li, Feng-Feng

来源：Acta Biomaterialia, 2011, 7(2): 634-643.

DOI：10.1016/j.actbio.2010.09.011

• Nerve guidance conduit
• Peripheral nerve regeneration
• Silk fibroin
• Poly(L-lactic acid-co-epsilon-caprolactone)
• Electrospinning

Peripheral nerve regeneration remains a significant clinical challenge to researchers. Progress in the design of tissue engineering scaffolds provides an alternative approach for neural regeneration. In this study aligned silk fibroin (SF) blended poly(L-lactic acid-co-epsilon-caprolactone) (P(LLA-CL)) nanofibrous scaffolds were fabricated by electrospinning methods and then reeled into aligned nerve guidance conduits (NGC) to promote nerve regeneration. The aligned SF/P(LLA-CL) NGC was used as a bridge implanted across a 10 mm defect in the sciatic nerve of rats and the outcome in terms of of regenerated nerve at 4 and 8 weeks was evaluated by a combination of electrophysiological assessment and histological and immunohistological analysis, as well as electron microscopy. The electrophysiological examination showed that functional recovery of the regenerated nerve in the SF/P(LLA-CL) NGC group was superior to that in the P(LLA-CL) NGC group. The morphological analysis also indicated that the regenerated nerve in the SF/P(LLA-CL) NGC was more mature. All the results demonstrated that the aligned SF/P(LLA-CL) NGC promoted peripheral nerve regeneration significantly better in comparison with the aligned P(LLA-CL) NGC, thus suggesting a potential application in nerve regeneration.

• 出版日期2011-2
• 单位嘉兴学院; 东华大学