Updates in inducible transgene expression using viral vectors: from transient to stable expression

作者：Mortimer Cara L; Dugdale Benjamin; Dale James L^*

来源：Current Opinion in Biotechnology, 2015, 32: 85-92.

DOI：10.1016/j.copbio.2014.11.009

The prospect of economically producing useful biologics in plants has greatly increased with the advent of viral vectors. The ability of viral vectors to amplify transgene expression has seen them develop into robust transient platforms for the high-level, rapid production of recombinant proteins. To adapt these systems to stably transformed plants, new ways of deconstructing the virus machinery and linking its expression and replication to chemically controlled promoters have been developed. The more advanced of these stable, inducible hyper-expression vectors provide both activated and amplified heterologous transgene expression. Such systems could be deployed in broad acre crops and provide a pathway to fully exploit the advantages of plants as a platform for the manufacture of a wide spectrum of products.

• 出版日期2015-4