Alternative therapeutic advantages of catfish bile on atopic dermatitis: protection of T cell-mediated skin disease via antioxidant activities

作者：Lee Do Ik; Huh Yoon Joo; Hwang Kwang Woo; Choi YoungWook; Choi Jae Seok; Han Sam Yong; Gyoung Young Soo; Joo Seong Soo^*

来源：Journal of Pharmacy and Pharmacology, 2011, 63(10): 1327-1335.

DOI：10.1111/j.2042-7158.2011.01337.x

• 2,4-dinitrochlorobenzene
• antioxidant
• atopic dermatitis
• immunosuppressant
• Silurus asotus
• T helper 1/2 cytokines

Objectives In the present study, we aimed to examine the anti-atopic properties of bile from the cat fish, Silurus asotus, to determine its possible use as a pharmaceutical product.
Methods The anti-atopic activities of cat fish bile were examined in a non-cell antioxidant, in-vitro assay (splenocytes and mast cells) and a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like mouse model.
Results The results of these experiments revealed that Silurus asotus bile (SAB) scavenges radicals and protects proteins from superoxide attacks, suggesting that SAB suppresses the T helper (Th) type 2-skewed immune response. Th1/Th2 mRNA cytokines (interleukin (IL)-2, interferon (IFN)-gamma and IL-4) from mouse splenocytes were effectively inhibited, and the release of beta-hexosaminidase in RBL-2H3 mast cells was significantly suppressed by SAB. These results were supported by screening the Th1/Th2 cytokine mRNAs (IL-2, IFN-gamma and IL-4) from lymph nodes in DNCB-treated mice. More dramatic results were observed in the histological changes at higher SAB concentrations (5%) compared to the therapeutic control, visualized using hematoxylin-eosin (H&E) staining.
Conclusions The results presented in this study suggest that SAB may provide functional advantages with regard to treating atopic dermatitis because of its antioxidant and immunesuppressive effects.

• 出版日期2011-10