ANTIOXIDANT AND NEUROPROTECTIVE EFFECTS OF JP05, A POLYHERBAL MEDICINE, ON HYDROGEN PEROXIDE-INDUCED OXIDATIVE INJURY IN NEURO-2A NEUROBLASTOMA CELLS

作者：Mahesh Ramalingam; Jung Hyo Won; Kim Young Ho; Park Yong Ki^*

来源：Current Topics in Nutraceutical Research, 2011, 9(1-2): 13-23.

• Antioxidant
• Apoptosis
• MAPK
• Neuro-2a
• Neuroprotection
• NF-(e)over-capB

Herbal medicines with antioxidant properties are believed to have potential therapeutic effect against oxidative stress in neurodegenerative diseases. In this study, we evaluated the antioxidant and neuroprotective effects of JP05, a polyherbal medicine, on H(2)O(2)-induced oxidative injury in Neuro-2a (N2a) cells. The cells were treated with or without JP05, and then stimulated with 500 mu M H(2)O(2). Cell viability was determined by MTT assay, and the releases of NO and LDH and intracellular ROS were measured. The levels of MDA and PCO, the activities of SOD, CAT and GPx, and contents of GSH and GSSG were assayed with respect to expressions of iNOS, nNOS, Bax and Bcl-2 via the NF kappa B and MAPK pathways. JP05 potently inhibited the O(2)(center dot-), H(2)O(2), OH(center dot), NO(center dot) and possessed metal chelating properties. JP05 improved cell survival decreased the levels of NO, LDH and intracellular ROS, modulated antioxidants by suppressing the expression of iNOS, nNOS and Bax, and increased Bcl-2 expression by modulating the NF-kappa B and MAPK pathways. Our results indicate that JP05 protects neuronal cells against H(2)O(2)-induced oxidative injury by preventing cell apoptotic cascades and modulating NF-kappa B and MAPK pathways, suggesting that JP05 has therapeutic potential for the treatment of neurodegenerative diseases.

• 出版日期2011-5